Powerful and most sensitive HTS plate reader

The PHERAstar® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, and dedicated AlphaScreen® and TRF lasers, the PHERAstar FSX will perform your assay with ease and speed, even in 3456-well microplates.

Technical Specification

Detection modes

Fluorescence intensity – incl. FRET

Fluorescence polarization


Luminescence (flash and glow) – incl. BRET

Time-resolved fluorescence


UV/Vis absorbance spectra
Measurement modes

Top and bottom reading

Endpoint and kinetic

Sequential multi-excitation

Sequential multi-emission

Simultaneous Dual Emission

Spectral scanning (absorbance)

Real-time ratiometric measurements

Well scanning
Microplate formats

Up to 3456-well plates, user-definable

LVis Plate with 16 low volume microspots (2 μL))

Two matched pairs of photomultiplier tubes (PMTs), optimized for different detection modes

CCD spectrometer
Wavelength selection

Optic Modules: Up to six application-specific modules (includes all filters, dichroics, etc. for an application) 

For top and bottom reading

UV/vis absorbance spectrometer: Full spectra or 8 distinct wavelengths in < 1 sec / well
Spectral range

Filters    230 – 750 nm or 230 – 900 nm for FI, FP

230 – 750 nm for LUM

230 – 900 nm for TRF

Spectrometer    220 – 1000 nm for ABS
Read times
Flying mode
(1 flash) 

14 s (384)
27 s (1536) 

10 flashes38 s (384)
1 min 52 s (1536) 
50 flashes1 min 29 s (384)
5 min 18 s (1536) 
Linear, orbital, and double-orbital with user-defi nable time and speed
+5 °C above ambient up to 45 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer